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pMD18-Control质粒

货号：kl-zl-0175-01

货号	产品名称	规格	库存	价格	数量	购买
kl-zl-0175-01	pMD18-Control质粒	NA		立即咨询	+ -

产品介绍
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pMD18-Control质粒货号：kl-zl-0175

启动子：	Lac/lac
复制子：	ColE1 ori
质粒分类：	大肠杆菌载体；克隆质粒
质粒大小：	2692bp
质粒标签：	LacZ
原核抗性：	氨苄青霉素Amp
克隆菌株：	DH5α
培养条件：	37℃，有氧，LB
5'测序引物：	M13R：CAGGAAACAGCTATGACC
3'测序引物：	M13F：TGTAAAACGACGGCCAGT
备注：	制备T载体

质粒简介：
pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for this Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
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