pMD2.G质粒 货号：kl-zl-0563 规格：20ul
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启动子： |
CMV |
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复制子： |
pUC ori |
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终止子： |
beta-globin poly(A) |
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质粒分类： |
病毒系列，慢病毒包装载体 |
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质粒大小： |
5822bp |
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质粒标签： |
VSV-G |
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原核抗性： |
Amp |
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克隆菌株： |
Stbl3 |
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培养条件： |
37℃，有氧 LB |
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表达宿主： |
哺乳细胞 |
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诱导方式： |
无须诱导，瞬时表达 |
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5'测序引物： |
CMV-F：CGCAAATGGGCGGTAGGCGTG |
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3'测序引物： |
根据序列设计引物 |
质粒简介：
PMD2. belong to the VSV-G expression plasmid, and can be used together with pSPAX2.
图谱：
序列：
ORIGIN
1 tggaggcaag gcctgcaaaa tgcaatactg caagcattgg ggagtcagac tcccatcagg
61 tgtctggttc gagatggctg ataaggatct ctttgctgca gccagattcc ctgaatgccc
121 agaagggtca agtatctctg ctccatctca gacctcagtg gatgtaagtc taattcagga
181 cgttgagagg atcttggatt attccctctg ccaagaaacc tggagcaaaa tcagagcggg
241 tcttccaatc tctccagtgg atctcagcta tcttgctcct aaaaacccag gaaccggtcc
301 tgctttcacc ataatcaatg gtaccctaaa atactttgag accagataca tcagagtcga
361 tattgctgct ccaatcctct caagaatggt cggaatgatc agtggaacta ccacagaaag
421 ggaactgtgg gatgactggg caccatatga agacgtggaa attggaccca atggagttct
481 gaggaccagt tcaggatata agtttccttt atacatgatt ggacatggta tgttggactc
541 cgatcttcat cttagctcaa aggctcaggt gttcgaacat cctcacattc aagacgctgc
601 ttcgcaactt cctgatgatg agagtttatt ttttggtgat actgggctat ccaaaaatcc
661 aatcgagctt gtagaaggtt ggttcagtag ttggaaaagc tctattgcct cttttttctt
721 tatcataggg ttaatcattg gactattctt ggttctccga gttggtatcc atctttgcat
781 taaattaaag cacaccaaga aaagacagat ttatacagac atagagatga accgacttgg
841 aaagtaactc aaatcctgca caacagattc ttcatgtttg gaccaaatca acttgtgata
901 ccatgctcaa agaggcctca attatatttg agtttttaat ttttatgaaa aaaaaaaaaa
961 aaaacggaat tcaccccacc agtgcaggct gcctatcaga aagtggtggc tggtgtggct
1021 aatgccctgg cccacaagta tcactaagct cgctttcttg ctgtccaatt tctattaaag
1081 gttcctttgt tccctaagtc caactactaa actgggggat attatgaagg gccttgagca
1141 tctggattct gcctaataaa aaacatttat tttcattgca atgatgtatt taaattattt
1201 ctgaatattt tactaaaaag ggaatgtggg aggtcagtgc atttaaaaca taaagaaatg
1261 aagagctagt tcaaaccttg ggaaaataca ctatatctta aactccatga aagaaggtga
1321 ggctgcaaac agctaatgca cattggcaac agcccctgat gcctatgcct tattcatccc
1381 tcagaaaagg attcaagtag aggcttgatt tggaggttaa agttttgcta tgctgtattt
1441 tacattactt attgttttag ctgtcctcat gaatgtcttt tcactaccca tttgcttatc
1501 ctgcatctct cagccttgac tccactcagt tctcttgctt agagatacca cctttcccct
1561 gaagtgttcc ttccatgttt tacggcgaga tggtttctcc tcgcctggcc actcagcctt
1621 agttgtctct gttgtcttat agaggtctac ttgaagaagg aaaaacaggg ggcatggttt
1681 gactgtcctg tgagcccttc ttccctgcct cccccactca cagtgacccg gaatccctcg
1741 acatggcagt ctagcactag tgcggccgca gatctgcttc ctcgctcact gactcgctgc
1801 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
1861 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
1921 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
1981 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
2041 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
2101 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
2161 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
2221 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
2281 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
2341 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
2401 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
2461 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
2521 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
2581 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
2641 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
2701 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
2761 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
2821 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
2881 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
2941 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
3001 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
3061 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
3121 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
3181 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
3241 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
3301 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
3361 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
3421 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
3481 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
3541 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
3601 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
3661 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtggat cccctgaggg
3721 ggcccccatg ggctagagga tccggcctcg gcctctgcat aaataaaaaa aattagtcag
3781 ccatgagctt ggcccattgc atacgttgta tccatatcat aatatgtaca tttatattgg
3841 ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt aatagtaatc
3901 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
3961 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
4021 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
4081 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
4141 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
4201 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
4261 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
4321 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
4381 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
4441 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
4501 cctccataga agacaccggg accgatccag cctcccctcg aagcttacat gtggtaccga
4561 gctcggatcc tgagaacttc agggtgagtc tatgggaccc ttgatgtttt ctttcccctt
4621 cttttctatg gttaagttca tgtcatagga aggggagaag taacagggta cacatattga
4681 ccaaatcagg gtaattttgc atttgtaatt ttaaaaaatg ctttcttctt ttaatatact
4741 tttttgttta tcttatttct aatactttcc ctaatctctt tctttcaggg caataatgat
4801 acaatgtatc atgcctcttt gcaccattct aaagaataac agtgataatt tctgggttaa
4861 ggcaatagca atatttctgc atataaatat ttctgcatat aaattgtaac tgatgtaaga
4921 ggtttcatat tgctaatagc agctacaatc cagctaccat tctgctttta ttttatggtt
4981 gggataaggc tggattattc tgagtccaag ctaggccctt ttgctaatca tgttcatacc
5041 tcttatcttc ctcccacagc tcctgggcaa cgtgctggtc tgtgtgctgg cccatcactt
5101 tggcaaagca cgtgagatct gaattctgac actatgaagt gccttttgta cttagccttt
5161 ttattcattg gggtgaattg caagttcacc atagtttttc cacacaacca aaaaggaaac
5221 tggaaaaatg ttccttctaa ttaccattat tgcccgtcaa gctcagattt aaattggcat
5281 aatgacttaa taggcacagc cttacaagtc aaaatgccca agagtcacaa ggctattcaa
5341 gcagacggtt ggatgtgtca tgcttccaaa tgggtcacta cttgtgattt ccgctggtat
5401 ggaccgaagt atataacaca ttccatccga tccttcactc catctgtaga acaatgcaag
5461 gaaagcattg aacaaacgaa acaaggaact tggctgaatc caggcttccc tcctcaaagt
5521 tgtggatatg caactgtgac ggatgccgaa gcagtgattg tccaggtgac tcctcaccat
5581 gtgctggttg atgaatacac aggagaatgg gttgattcac agttcatcaa cggaaaatgc
5641 agcaattaca tatgccccac tgtccataac tctacaacct ggcattctga ctataaggtc
5701 aaagggctat gtgattctaa cctcatttcc atggacatca ccttcttctc agaggacgga
5761 gagctatcat ccctgggaaa ggagggcaca gggttcagaa gtaactactt tgcttatgaa
5821 ac
